Comment by shpongled

Comment by shpongled 2 days ago

4 replies

Close - this is a SDS-PAGE gel, and you run it using proteins. The bands in the first two rows are from a western blot (gel is transferred to a membrane), where you use antibodies against those specific proteins to detect them. The Pon S row is Ponceau S, a dye that non-specifically detects all proteins - so it's used as a loading control, to make sure that the same amount of total protein is loaded in each lane of the gel.

doctorpangloss 2 days ago

Is it conceivable that the control was run once because the key result came from the same run? I can see a reviewer asking for it in all three figures, whereas they may drafted it only in one

  • gus_massa 2 days ago

    The horizontal label is fine, it says Pon S in all images. (I guess a wrong label would be obvious to detect for specialists.)

    The problem are the vertical labels

    In Figure 1e it says: "MT1+2", "MT2" and "MT1"

    In Figure 3a it says: "5'-CR1", "CR2" and "3'-UTR"

    In Figure 3b it says: "CR2", "CR3" and "CR4"

    • [removed] 2 days ago
      [deleted]
  • shpongled 2 days ago

    Based on the images, it is inconceivable that these are from the same run (see the dramatically different levels of TRF-S in each gel. One column/lane = one sample). This isn't something that would be included because of a reviewer - loading controls are required to meaningfully interpret the results (e.g. the data is useless without such a control).