Comment by shpongled
Close - this is a SDS-PAGE gel, and you run it using proteins. The bands in the first two rows are from a western blot (gel is transferred to a membrane), where you use antibodies against those specific proteins to detect them. The Pon S row is Ponceau S, a dye that non-specifically detects all proteins - so it's used as a loading control, to make sure that the same amount of total protein is loaded in each lane of the gel.
Is it conceivable that the control was run once because the key result came from the same run? I can see a reviewer asking for it in all three figures, whereas they may drafted it only in one