Comment by doctorpangloss 2 days ago
Is it conceivable that the control was run once because the key result came from the same run? I can see a reviewer asking for it in all three figures, whereas they may drafted it only in one
Based on the images, it is inconceivable that these are from the same run (see the dramatically different levels of TRF-S in each gel. One column/lane = one sample). This isn't something that would be included because of a reviewer - loading controls are required to meaningfully interpret the results (e.g. the data is useless without such a control).
The horizontal label is fine, it says Pon S in all images. (I guess a wrong label would be obvious to detect for specialists.)
The problem are the vertical labels
In Figure 1e it says: "MT1+2", "MT2" and "MT1"
In Figure 3a it says: "5'-CR1", "CR2" and "3'-UTR"
In Figure 3b it says: "CR2", "CR3" and "CR4"