Comment by olivia-banks

Comment by olivia-banks 4 hours ago

0 replies

I'm not sure if I understand your comment, but they were able to grow and propagate scHPXV in their lab. Link the sequence? Sure, it's on NCBI

https://www.ncbi.nlm.nih.gov/nuccore/NC_001611.1

As for getting the nucleotides themselves, there are numerous services for ordering oligonucleotides which you can "stitch together." I think this used to be done with phosphoramidite synthesis, but the article I linked says they used plasmid synthesis, and ordered from ThermoFisher.

https://www.thermofisher.com/us/en/home/life-science/cloning...

I'm not sure what the price would be on this (I would imagine very high?), but it has to be cheaper than phosphoramidite chemistry. Nevertheless, the price of doing this sort of things w/ plasmids is plummeting.