koeng 7 hours ago

I really need to do a write-up. I kinda just whip up the easiest path and do it.

For example for the grape, I needed to knock out some tryptophan synthesis genes so I could redirect the bioflux. Problem is that in bakers yeast they have a whole buncha copies of their chromosomes, so I had to knock out one of the genes and replace it with a different gene from grapes. Did that with a quick lil CRISPR switch.

Had to electroporate tho because the transformation rates on wild/bakers/non-lab yeast are so garbage

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  • amarant 5 hours ago

    How much expensive lab equipment do you need to do something like this?

    "A quick lil CRISPR switch" sounds like "oh just my homemade fusion reactor hooked up to my kitchen warp drive" to me, yet you make it sound so simple!

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  • Hammerhead12321 6 hours ago

    Starting from nothing, how much time (and money) might it take an individual to learn how to be able to do these things?

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