Interesting concept, but between the broken hardware and the way they gave up before getting anything useful this article was rather disappointing:
> Another problem was our flow cell was malfunctioning from the start — only 623 out of 2048 pores were working.
Is this normal for the machine? Is there a better write up somewhere where they didn’t give up immediately after one attempt?
Do you have a write up somewhere? If not, it would be amazing if you wrote one!
I was planning on doing a similar thing (also with saliva) once I finished moving in and had a bit more time after conferences. (But, of course, I’d have to go through and actually figure out all of the mechanics and so on.)
I think it was pretty interesting in a "what would likely happen if you tried this" way. Negative results are good. A lot of technical problems is what I'd expected though, from my little experience in genetic genealogy.
Hi, believe it or not, I have actually done what the authors were attempting. I used saliva rather than blood as a source of DNA and extracted it using a Qiagen kit.
My Nanopore flow cell had nearly every pore working from the start. So I would say that is not normal. Maybe it was stored incorrectly.